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Alstem Inc
mouse ipsc line  Mouse Ipsc Line, supplied by Alstem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/mouse ipsc line/product/Alstem Inc Average 90 stars, based on 1 article reviews
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2026-03
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Journal: Stem cell research
Article Title: Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11 _1260Ins(TT)
doi: 10.1016/j.scr.2022.102728
Figure Lengend Snippet: Characterization of the Tex11_1260Ins(TT) induced pluripotent stem cell (iPSC) line derived from an infertile male mouse model. (A) Phase contrast image showing normal morphology of Tex11_1260Ins(TT) iPSCs growing on a feeder-coated plate (scale bar = 50 μm). (B) Genotyping PCR products of Tex11_1260Ins(TT) iPSCs to confirm their origin from Tex11_1260Ins(TT) mouse fibroblasts. Wild-type control iPSCs (labeled as (+) WT) were used as a control. (C) Sanger sequencing of PCR products for wild type iPSCs, Tex11_1260Ins(TT) fibroblasts , and Tex11_1260Ins(TT) iPSCs to confirm their genotype as wild-type (GATG) or mutant (TTGGTA). (D) PCR results confirming the sex of Tex11_1260Ins(TT) iPSCs as male using F Sry -R Sry primer pair specific for the Sry gene. Cells of male (M) origin or female (F) origin were used as controls. Fc-Rv primer pair was used to confirm presence of DNA in all cell samples. (E) RT-PCR result showing absence of sendai virus (SeV) in Tex11_1260Ins(TT) iPSCs at passage 16. Tex11_1260Ins(TT) iPSCs at passage 3 were used as positive control for SeV. GAPDH housekeeping gene was used as a control for RT-PCR analysis. (F) Tex11_1260Ins(TT) iPSCs showing normal karyotype of 40 chromosomes (mouse). (G) Immunofluorescent staining of Tex11_1260Ins(TT) iPSCs showing positive staining for pluripotent markers Alkaline phosphatase (AP), SSEA1, SOX2, OCT4, NANOG, DPPA2. (H) Flow cytometry analysis showing no difference in percentage of SSEA1+, SOX2+, or OCT4 + Tex11_1260Ins(TT) iPSCs compared to control iPSCs. (I) Teratoma assay analysis confirming tri-lineage (endoderm, mesoderm, ectoderm) differentiation potential of transplanted Tex11_1260Ins(TT) iPSCs.
Article Snippet: A published
Techniques: Derivative Assay, Control, Labeling, Sequencing, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Virus, Positive Control, Staining, Flow Cytometry
Journal: Stem cell research
Article Title: Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation Tex11 _1260Ins(TT)
doi: 10.1016/j.scr.2022.102728
Figure Lengend Snippet: Characterization and validation.
Article Snippet: A published
Techniques: Biomarker Discovery, Immunocytochemistry, Staining, Flow Cytometry, Mutagenesis, Sequencing, Amplification, Positive Control, Negative Control, Derivative Assay, Plasmid Preparation, Modification, Immunohistochemistry